What is the difference between spectral flow cytometry and traditional flow cytometry?

Spectral flow cytometry allows us to analyze the full fluorescence spectrum, which enables differentiation between fluorochromes that would normally be indistinguishable in traditional flow cytometry. While traditional flow cytometry generally requires some compensation to clearly differentiate between different populations, spectral flow cytometry requires almost no compensation, decreasing the risk of error and false positives and negatives.1 Learn more about spectral flow cytometry for characterization.

  Conventional Flow Cytometry Spectral Flow Cytometry with Certis
Colors Up to 18 colors Up to 24 colors
Detector per fluorochrome 1 38
Spectrum analyzed Narrow bandwidth analysis Entire spectral profile analysis
Able to distinguish overlapping fluorochromes Difficult Accurate distinction
Compensation needed? Yes No/very little
Detector sensitivity Standard Higher – able to evaluate expression of low-expressed antigens

1 Sanders CK, Mourant JR. Advantages of Full Spectrum Flow Cytometry. J Biomed Opt. 2013;18(3):037004.