When creating PDX models, does Certis implant tumor fragments or single-cell suspensions?

Certis implants both tumor chunk and single-cell suspensions, depending on the circumstances. Certis implants tumor chunks of approximately 2-3mm when possible, as this technique helps preserve the original tumor’s histological architecture and stroma construction, cell-cell adhesions and cell type composition. Orthotopic engraftment further improves clinical relevance through recapitulation of the tumor microenvironment. If implantation of a tumor chunk is not possible, we use single-cell suspensions. Currently, single- cell suspensions are used for brain, bone and lung model development.