In vitro functional assays characterize tumor cell behavior, aggressiveness, and microenvironmental support mechanisms in response to therapeutic interventions. Capabilities include assessment of cell viability, apoptosis, cell cycle progression, invasive potential, migratory capacity, stem-like properties, metastatic behavior, and angiogenic responses through luminescent assays, irradiation studies, live cell imaging, migration tracking, and 3D spheroid/sphere-forming cultures.